Multidimensional Digital Bioassay Platform Based on an Air-Sealed Femtoliter Reactor Array Device

Single-molecule experiments have been helping us to get deeper inside biological phenomena by illuminating how individual molecules actually work. Digital bioassay, in which analyte are individually confined small compartments be analyzed, is an emerging technology single-molecule biology and applies various entities (e.g., cells virus particles). However, digital bioassay not compatible with multiconditional multiparametric assays, hindering in-depth understanding of analytes. This because current lacks a repeatable solution-exchange system that keeps analytes compartments. To address this challenge, we developed platform easy solution exchanges, called multidimensional (MD) bioassay. We immobilized single arrayed femtoliter (10–15 L) reactors sealed them airflow. The each reactor was stable showed no cross-talk via leakage for more than 2 h, over 30 rounds perfect exchanges were successfully performed. With assays based on our system, could quantitatively determine inhibitor sensitivities influenza A particles alkaline phosphatase (ALP) molecules, has never achieved conventional bioassays. Further, demonstrated ALPs from two origins can precisely distinguished assay also difficult Thus, MD versatile gain insight into unprecedented resolution.